Poly-L-lysine amplification of protamine immobilization and heparin adsorption

✍ Scribed by Zhang, Yuehua ;Singh, Vijendra K. ;Yang, Victor C.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 220 KB
• Volume: 42
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199811)42:2<182::aid-jbm3>3.0.co;2-l

✦ Synopsis

We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin-and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method to augment protamine loading on the fiber, as well as to create multiple layers of immobilized protamine for heparin interaction. Results show that such a method yielded about a threefold increase in protamine loading and, consequently, about a fourfold enhancement in heparin adsorption when compared with the control without poly-L-lysine amplification. This technological improvement may facilitate development of a new generation of protamine filters with capacity and efficacy suitable for various clinical applications in extracorporeal heparin removal.