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Poly a blocks in the nuclear ribonucleoprotein complexes, containing pre-mRNA

✍ Scribed by O. P. Samarina; N. A. Aitkhozhina; J. Besson

Publisher: Springer
Year: 1973
Tongue: English
Weight: 323 KB
Volume: 1
Category: Article
ISSN: 0301-4851
DOI: 10.1007/bf00357641

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✦ Synopsis

Poly A was found in nuclear particles containing pre-mRNA. It was shown that during the isolation of 30S particles from rat liver or Ehrlich ascites carcinoma nuclei, all poly A is detached from the particles containing pre-mRNA and is found in the form of RNP with a sedimentation coefficient of about 14S. When RNP polyparticles are isolated in the presence of RNase inhibitor poly A is distributed among the particles of higher molecular weights.Since the sedimentation properties and buoyant density of the poly A-containing particles are different from the 30S particles it was suggested that the poly A fragments are bound not with informofers, but with another kind of protein.

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