Polarographic determination of superoxide dismutase

✍ Scribed by Adelio Rigo; Paolo Viglino; Giuseppe Rotilio

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 400 KB
• Volume: 68
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90672-7

✦ Synopsis

A polarographic procedure is described which allows determination of the catalytic constants for superoxide dismutase-catalyzed reactions. The method presents a single and rapid evaluation of the enzyme concentrations as well as determination of its activity under different conditions; e.g., pH between 9 and 13, presence of urea, guanidine, sodium dodecyl sulphate and inhibitors such as CN-and N3-.

The results fit very well with data previously obtained with other methods and show that this polarographic procedure can be used under conditions that render the other methods unsuitable for the measurement of the enzyme activity.