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Polarized secretion of IGF-I and IGF-I binding protein activity by cultured aortic endothelial cells

✍ Scribed by W. Robert Taylor; Robert M. Nerem; R. Wayne Alexander

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
493 KB
Volume
154
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Insulin‐like growth factor‐I (IGF‐I) secretion by the vascular endothelium has been proposed to play a role in the regulation of vascular smooth muscle cell proliferation. Because vascular smooth muscle cells are adjacent to the abluminal surface of the endothelium, we tested the hypothesis that secretion of IGF‐I by endothelial cells is polarized. Porcine aortic endothelial cells were cultured on permeable membranes and IGF‐I measured by radioimmunoassay. Basal secretion exceeded apical secretion by a ratio of 2.3 ± 0.2:1.0 (P < 0.05). We also identified 35 kDa IGF‐I binding protein activity that is preferentially secreted on the basal surface of endothelial cells. We conclude that both IGF‐I and IGF‐I binding protein activity secretion by endothelial cells is polarized towards the basal surface of the endothelium. A polarized secretion mechanism for IGF‐I may be of importance in the normal growth and differentiation of the vasculature as well as in the development of vascular pathology. © 1993 Wiley‐Liss, Inc.

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