Ploidy assessment of benign and malignant ovarian tumors by flow cytometry. A clinicopathologic study

✍ Scribed by Ole-Erik Iversen; Elsa Skaarland

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 562 KB
• Volume: 60
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19870701)60:1<82::aid-cncr2820600114>3.0.co;2-d

✦ Synopsis

In a prospective study, 112 fresh ovarian tumor samples were collected from 83 consecutive patients. Cellular DNA content was measured by flow cytometry. All the benign (n = 24) and semimalignant (n = 6) tumors were diploid. Of 50 malignant tumors, 24 (48%) were diploid and 26 (52%) were aneuploid.

Aneuploidy was more frequent in the advanced stages of the disease, in tumors of low degree of differentiation, and in older patients. The patients with aneuploid tumors had smaller primary tumors and more often ascites. The fraction of cells with Sphase DNA content was higher in the aneuploid tumors. No association was seen to the tumor type. Ploidy determination is objective and reproducable.

Aneuploidy associates to most negative prognostic factors in ovarian carcinoma and may reflect the aggressiveness of the tumor. The ploidy status may be taken into consideration in the stratification of patients of comparable risk for treatment studies.

Cancer 60:82-87. 1987.

VARIAN CARCINOMA has been reported to occur 0 with increasing incidence in a number of coun-In Norway it is now the most frequently reported gynecologic malignancy. The number of deaths per year from this disease equals the number of deaths from all other gynecologic malignancies added together. Symptoms are vague and uncharacteristic and no diagnostic procedure for early detection is available. The diagnosis 84