Platinum determination in cis-dichlorodiammineplatinum(II)-DNA complexes by differential pulse polarography

✍ Scribed by Oldřich Vrána; Viktor Brabec

• Book ID: 102987158
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 684 KB
• Volume: 142
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90510-4

✦ Synopsis

A simple polarogmphic assay for platinum determination in cis-dichlorodiammineplatinum(II)-DNA complexes is described. The method makes it possible to determine the free (unbound) drug in the presence of DNA or platinum-DNA complex, i.e., without a separation of free drug and macromolecular components of the solution to be analyzed. This method is based on the polarographic activity of intact cisdichlorodiammineplatinum( 11) at -1.5 V, which can be measured by differential pulse polarography even in the presence of DNA or platinum-DNA complex. The lower level of analytical utility of this method is ca. I X 10e6 M (195 ng Of phtinUm/ml).

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