Platform for Highly Sensitive Alkaline Phosphatase-Based Immunosensors Using 1-Naphthyl Phosphate and an Avidin-Modified Indium Tin Oxide Electrode
✍ Scribed by Md. Abdul Aziz; Kyungmin Jo; Md. Abdul Qaium; Chan-Hwa Huh; In Seok Hong; Haesik Yang
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 284 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Abstract
We report a versatile platform for highly sensitive alkaline phosphatase (ALP)‐based electrochemical biosensors that uses an avidin‐modified indium tin oxide (ITO) electrode as a sensing electrode and 1‐naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1‐naphthol (ALP product) on the ITO electrodes allow a high signal‐to‐background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.