Platelet-derived growth factor-induced arachidonic acid release for enhancement of prostaglandin E2 synthesis in human gingival fibroblasts pretreated with interleukin-1β
✍ Scribed by Sumi Nakao; Yorimasa Ogata; Yoshifumi Yamamoto; Shunsuke Furuyama; Hiroshi Sugiya
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 186 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Platelet-derived growth factor (PDGF) is a biological mediator for connective tissue cells and plays a critical role in a wide variety of physiological and pathological processes. We here investigated the effect of PDGF on arachidonic acid release and prostaglandin E 2 (PGE 2 ) synthesis in human gingival fibroblasts (HGF). PDGF induced arachidonic acid release in a time-and dose-dependent manner, and simultaneously induced a transient increase in intracellular Ca 2þ concentration ([Ca 2þ ] i ), but less provoked PGE 2 release and cyclooxygenase-2 (COX-2) mRNA expression. When [Ca 2þ ] i was increased by Ca 2þ -mobilizaing reagents, arachidonic acid release was increased. The PDGF-induced arachidonic acid release and increase in [Ca 2þ ] i were prevented by a tyrosine kinase inhibitor. On the other hand, in the HGF pre-stimulated with interleukin-1b (IL-1b), PDGF clearly increased PGE 2 release. The PDGFinduced PGE 2 release was inhibited by a tyrosine kinase inhibitor. In the HGF pretreated with IL-1b, arachidonic acid strongly enhanced PGE 2 release and COX-2 mRNA expression. These results suggest that PDGF stimulates arachidonic acid release by the increase in [Ca 2þ ] i via tyrosine kinase activation, and which contributes to PGE 2 production via COX-2 expression in HGF primed with IL-1b.