Platelet adhesion to novel phospholipid materials: Modified phosphatidylcholine covalently immobilized to silica, polypropylene, and PTFE materials
✍ Scribed by K�hler, A. S. ;Parks, P. J. ;Mooradian, D. L. ;Rao, G. H. R. ;Furcht, L. T.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 576 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Based on the premise of achieving blood compatibility through mimicking the chemical constituents of the biologically inert surface of the unactivated platelet membrane, a process was developed that entails the covalent grafting of modified phosphatidylcholine molecules to materials including silica, polypropylene, and polytetrafluoroethylene (PTFE) polymer films. These materials were characterized using x-ray photoelectron spectroscopy (XPS) and contactangle measurements. The phosphatidylcholine-containing materials (PC materials) were used as substrates in the platelet-adhesion assays and were subjected to enzymatic degradation evaluation. Phosphatidylcholine-grafted silica materials do not support platelet adhesion. In addition the number of adherent platelets correlate with the amount of grafted phospholipid present, as indicated by the phosphorus/ carbon ratio obtained by XPS analysis. Platelet adhesion to phosphatidylcholine-grafted polypropylene and PTFE was inhibited 80% and 90%, respectively, when compared with platelet adhesion to unmodified polypropylene and PTFE.