Platelet adherence and detachment with adsorbed fibrinogen: A flow study with a series of hydroxyethyl methacrylate-ethyl methacrylate copolymers using video microscopy

✍ Scribed by Feuerstein, Irwin A. ;Glenn McClung, W. ;Horbett, Thomas A.

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 1010 KB
• Volume: 26
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820260208

✦ Synopsis

The adhesion and detachment of platelets were studied on glass coatings of a series of copolymers of hydroxyethyl methacrylate (HEMA) a n d ethyl methacrylate (EMA) with preadsorbed fibrinogen. Observations of the interactions of acridineorange-labeled washed platelets with these surfaces from a flowing (500 s-' wall shear rate) suspension in Tyrode's solution containing albumin and red cells were made with epifluorescent video microscopy (EVM). In some cases preadsorbed materials were incubated for 24 h, during which little or no loss of protein occurred. Protein surface concentration, by itself, was a poor indicator of expected cell adhesion and morphology. Surface chemistry was a second impor-tant factor which must be considered. A third observation is that for the 100% EMA copolymer, 24 h of incubation led to a large reduction in platelet adhesion when compared to the 100% EMA material without incubation. For the 0% and 100% EMA polymers, the percentage of contacting platelets which adhere and detach is greater for the 24-h incubation cases than for those not incubated. These results led to the conclusion that our most hydrophilic surface favors adhesion with detachment, transient cell contact, over long-term adhesion, as does incubation of adsorbed protein. A brief discussion is presented of a possible connection between this behavior and platelet consumption in viuo for hydrogels.