Lissencephaly patients are born with severe brain malformations and suffer from recurrent seizures. LIS1, the gene mutated in isolated lissencephaly patients, is a subunit of the heterotrimeric cytosolic enzyme platelet-activating factor acetylhydrolase (PAF-AH), interacts with tubulin, and affects
Platelet-activating factor acetylhydrolase (PAF-AH) in human kidney
β Scribed by Smaragdi Antonopoulou; Constantinos A. Demopoulos; Christos Iatrou; George Moustakas; Panos Zirogiannis
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 483 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0020-711X
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## Abstract Plateletβactivating factor (1β__O__βalkylβ2βacetylβ__sn__βglyceroβ3βphosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and
We have described the intracellular type of platelet-activating factor acetylhydrolase (PAF-AH) in the membrane extract of human red blood cells (RBCs). The enzymatic activity was inhibited by diisopropylfluorophosphate, trypsin or pronase E, but not affected by EDTA or the addition of 1 -0-hexadecy
Clinical lots of recombinant human platelet-activating factor acetylhydrolase (rhPAF-AH) were prepared in a lyophilized formulation. After reconstitution with sterile water for injection to form an aqueous solution (10 mM sodium citrate, 7.5 w/v% sucrose, and 0.1 w/v% Pluronic-F68, pH 6.5), a few vi