Platelet activating factor-acetylhydrolase in insects: Identification and partial characterization of a 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine acetylhydrolase in a cell-free system of Heliothis
✍ Scribed by Dr. Edward N. Lambremont; Boyd Malone; Fred Snyder
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 588 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0739-4462
No coin nor oath required. For personal study only.
✦ Synopsis
A specific acetylhydrolase that inactivates platelet activating factor (PAF; 1alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent cellular mediator in mammalian cells, by removal of the sn-2 acetyl moiety, has been found in the cytosolic fraction of several postemhryonir developmental stages and specific tissues of the corn eanvorm, Heliothis zea (Boddie). Effects of magnesium, calcium, EGTA, deoxycholate, dithiothreitol, diisopropylfluorophosphate, egg phosphatidylcholine, and an acylacetyl-glycerophosphocholine show that hydrolysis of the acetate moiety is due t o a specific acetylhydrolase for PAF. The activity does not appear to be due t o a typical cellular phospholipase A2 that utilizes phospholipid substrates with a long-chain acyl group at position sn-2 o f glycerol. Specific activities and properties of the acetylhydrolase from this insect match closely with those described from tissues of vertebrate animals.