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Plastein synthesis by α-chymotrypsin immobilized on hydrophobic agarose gel

✍ Scribed by Isao Karube; Yoichi Yugeta; Shuichi Suzuki


Publisher
Elsevier Science
Year
1980
Weight
389 KB
Volume
9
Category
Article
ISSN
0304-5102

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Effect of water-miscible aprotic solvent
✍ Pedro Lozano; Teresa Diego; José L. Iborra 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 353 KB

Immobilized a-chymotrypsin was used as catalyst to synthesize a kyotorphin derivative (Bz-Tyr-Arg-OEt) in the presence of five water-miscible aprotic solvents (dimethylsulphoxide, dimethylformamide, acetonitrile, acetone and tetrahydrofurane) at 30 °C. By using a kineticallycontrolled approach, the