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Plasmids controlling synthesis of hemolysin inEscherichia coli

โœ Scribed by Royer-Pokora, Brigitte ;Goebel, Werner


Publisher
Springer
Year
1976
Tongue
English
Weight
653 KB
Volume
144
Category
Article
ISSN
0026-8925

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As a first approach towards studying the regulation of hemolysin synthesis in Escherichia coli, we have fused lacZ into the four hly genes (hlyC, hlyA, hlyBa and hlyBb) using the Mud-1 (Mu::lacZ, Y, Apr) phage. The sites of insertion of Mud-1 within the various hly genes of the Hly plasmid pHly152 w

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Mitomycin C treatment of Escherichia coli cells containing one of the ColE plasmids results in specific inhibition of chromosomal protein synthesis and a high rate of protein synthesis from about 35% of the plasmid genome, whereas similar treatment of plasmid-free cells has no measureable effect on