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Plasmid-mediated isolation of a repressor protein

✍ Scribed by Raju K. Koduri; James Matthews; Joseph S. Gots

Book ID
102627000
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
474 KB
Volume
105
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

Protein-mediated isolation of plasmid DN
Protein-mediated isolation of plasmid DNA by a zinc finger-glutathione S-transferase affinity linker
✍ John Woodgate; David Palfrey; David A. Nagel; Anna V. Hine; Nigel K. H. Slater πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 200 KB

## Abstract The sequence‐specific affinity chromatographic isolation of plasmid DNA from crude lysates of __E. coli__ DH5Ξ± fermentations is addressed. A zinc finger‐GST fusion protein that binds a synthetic oligonucleotide cassette containing the appropriate DNA recognition sequence is described. T