✦ LIBER ✦
Plasmid mediated chromate resistance and reduction inPseudomonas mendocinaMCM B-180
✍ Scribed by P. K. Dhakephalkar; J. V. Bhide; K. M. Paknikar
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 290 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
✦ Synopsis
A chromate-resistant strain of Pseudomonas mendocina MCM B-180 capable of reducing hexavalent chromium was found to harbour a single plasmid. Incubation of the strain at 42°C for 24 h caused loss of chromate resistance as well as the plasmid, pARII80. Transformation of E. coli DH5ct with purified pARII80 plasmid DNA resulted in simultaneous acquisition of resistance to chromate and the appearance of plasmid in the transformants. Most importantly, the plasmid transfer was found to confer chromate reduction ability on to the E. coli transformants.