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Plasmid DNA Delivery by d-Alanine-Deficient Listeria monocytogenes

✍ Scribed by Benjamin E. Simon; Noel Ybarra; Aimée O. Bonneval; Ronald A. Barry

Book ID
109387475
Publisher
American Institute of Chemical Engineers
Year
2008
Tongue
English
Weight
298 KB
Volume
22
Category
Article
ISSN
8756-7938

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✍ Wuenscher, Michael D. ;Köhler, Stephan ;Goebel, Werner ;Chakraborty, Trinad 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 782 KB

A plasmid integration technique was developed for insertional inactivation of chromosomal Listeria monocytogenes genes. A Listeria-Escherichia coli shuttle vector (pLSV1) was constructed which carried the temperature-sensitive gram-positive replication origin from plasmid pTV32(Ts). An internal frag