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Plasmid burden in chemostat culture of Escherichia coli: Its effect on the selection for overproducers of host enzymes

✍ Scribed by P. Kyslik; M. Dobisova; H. Maresova; L. Sobotkova

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 503 KB
Volume: 41
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260410306

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✦ Synopsis

The effect of plasmid-mediated metabolic burden on the expression of the host genes and its consequences on the plasmid maintenance were studied in carbon-limited chemostat culture of Escherichia coli IEA(pBR322) subject to selection for strains overproducing chromosomally coded ribitol dehydrogenase. The chemostat population became rapidly heterogeneous and the competition among evolved strains was found to be crucial for the kinetics of the plasmid loss from the culture. The selective disadvantage in growth rate associated with plasmid carriage in the parentlike and ribitol dehydrogenase-overproducing strains was estimated.

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