✦ LIBER ✦

Plasmalogens and their oxidative degradation products in low and high density lipoprotein

✍ Scribed by Wolfgang Jira; Gerhard Spiteller

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 307 KB
Volume: 79
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(95)02511-1

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✦ Synopsis

The kinetics of tile autoxidation reaction of LDL and HDL with Fe 2 +/ascorbate was investigated. Aldehydes derived from plasrnalogens were determined by conversion to 2-alkyl-l,3-dithiolanes. Their oxidation products, 2-hydroxy-aldehydes, were trapped by reaction with pentafluorobenzylhydroxylamine. After derivatisation, the pentafluorobenzyloximes could be separated from other compounds by thin-layer chromatography. They were detected by gas chromatography applying an electron capture detector. LDL plasmalogens are oxidized nearly completely after 180 min while HDL plasmalogens suffer oxidation only to 5%. Oxidation of linoleic and arachidonic acid was investigated by simultaneous determination of 2-hydroxyheptanal.

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