Plasma β-N-acetylhexosaminidase isoenzyme composition and temperature conversion factors
✍ Scribed by J. Antonio Casal; Luis F. Pérez; J. Carlos Tutor
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 59 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Using the chromogenic substrate 3,3'-dichlorophenylsulfonphthaleinyl-N-acetyl-beta-D-glucosaminide for the activity determination of plasma beta-N-acetylhexosaminidase (Hex), the temperature conversion factors (TCF) offer a highly significant positive correlation with the relative proportion of Hex B isoenzyme (P< 0.001). The calculation of TCF 37 degrees/25 degrees C allows the isoenzyme composition of Hex to be determined quickly and cheaply. The results may be superimposed over those obtained in a previously described method based on the calculation of the enzyme's activation energy using four temperatures. However, the use of TCF 37 degrees/30 degrees C does not appear to comply with the required demands.