Plasma Protein Adsorption and Platelet Adhesion on Poly[bis(trifluoroethoxy)phosphazene] and Reference Material Surfaces
✍ Scribed by Alexander Welle; Michael Grunze; Dsidra Tur
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 557 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0021-9797
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✦ Synopsis
terials. However, the simple correlation between wetting
In this work we describe experiments designed to understand properties and blood compatibility cannot be generalized, the blood compatibility and resistance to platelet adhesion of because also the composition of the adsorbed protein film poly[bis(trifluoroethoxy)phosphazene] (Acta Polymerica 36, 627 will affect blood coagulation. (1985)) (PTFEP) coated surfaces. We compare quantitative in Adsorption and activation of coagulation-stimulating provitro protein adsorption measurements using enzyme linked imteins should be suppressed, whereas albumin adsorption is munosorbent assays (ELISA) and platelet adherence tests on known to ''passivate'' the surface of an implant. In general, PTFEP with other organic surfaces and hydroxylated glass. Com-
the physical and chemical parameters influencing blood copared to some materials of medical interest (polymethylmethacrylate and silicone) and other materials (hydroxylated glass, alde-agulation at artificial surfaces are not well understood, alhyde-, alkyl-, or amino-terminated surfaces) exhibiting a wide though some critical factors have been identified. A reduced range of physical properties, PTFEP showed the highest human platelet adherence and thrombus formation is correlated to serum albumin adsorption and the lowest adsorption of fibrinogen the smoothness of the implant (3), the presence of anionic and fibronectin. These proteins are related to thrombus formation surface charge (4), a balance of nondispersive/dispersive and cellular attachment, respectively. Coagulation-stimulating forces at the polymer/solution interface (5), and the mobilproteins are predominantly bound reversibly on PTFEP and do ity of polymer side chains (6) (cilia-concept to prevent any not appear to denaturate to the extent found on the other surfaces.
protein adsorption (7)).