Plasma membranes of muscle in experimental myotonia in rats

✍ Scribed by Irena Niebrój-Dobosz; Hubert Kwieciński; Konstanty Mrożek

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 704 KB
• Volume: 213
• Category: Article
• ISSN: 0340-5354
• DOI: 10.1007/bf00316276

✦ Synopsis

Determinations of protein and phospholipid composition, as well as enzymatic activity, were carried out in plasma membranes isolated from the muscle of rats, after different periods of 20,25-diazacholesterol administration. A decrease in the level of phospholipids, and in the total amount of plasma membrane proteins, connected with a relative reduction in the amount of protein of a molecular weight of 100000 daltons, was found. The activity of (Na+ + K+)-ATP-ase gradually decreased while a reverse tendency was observed in the case of 5'-nucleotidase. Changes in ATP-ase and phospholipids appeared even prior to electrophysiologically recorded signs of the myotonia. The mechanism of these changes and their possible role in myotonia are discussed.