Plasma Membrane Perturbation Induced by Organotins on Erythrocytes from Salmo irideus Trout

✍ Scribed by Giancarlo Falcioni; Rosita Gabbianelli; Anna Maria Santroni; Giovanna Zolese; David E. Griffiths; Enrico Bertoli

• Book ID: 102657989
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 522 KB
• Volume: 10
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199608)10:6<451::aid-aoc498>3.0.co;2-0

✦ Synopsis

Tributyltin chloride and its degradation products monobutyltin and dibutyltin act as water pollutants, owing to the use of tributyltin chloride as a biocide in marine paint formulations. These compounds are lipid-soluble and undergo bioaccumulation and bioconcentration. Sulno irideus trout erythrocytes were studied to evaluate the possible effects of these compounds on freshwater fish, which could be exposed to long-term effects due to bioaccumulation of organotins. Data showed that tributyltin increases the haemolysis rate, starting at 10 pM, while dibutyltin has a scant protective effect at each concentration tested. Similar studies were performed in the presence of carbon monoxide (CO), which is protective against membrane oxidative stress due to haemoglobin (Hb) auto-oxidation. In these conditions all the organotins tested induced an increase in the haemolysis rate. These results suggest that the consequence of auto-oxidation of Hb could condition the effects of some organotin compounds. Steadystate fluorescence of probes embedded in the lipidic part of the membrane was used to evaluate the modifications induced by organotins to the physico-chemical state of phospholipids.