Plasma membrane K+/H+-ATPase From leishmania donovani

✍ Scribed by Suping Jiang; Dr. Steven A. Anderson; G. Douglas Winget; Antony J. Mukkada

• Book ID: 102885118
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 663 KB
• Volume: 159
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041590109

✦ Synopsis

Abstract

Leishmania donovani has an active ^K+^/H^+^ exchange system on the surface membrane. Modulation of external K^+^ concentration resulted in a corresponding change in internal pH (pH~i~) suggesting a link between proton and potassium transport. Although a Na^+^/H^+^ antiporter is present on the plasma membrane, its sensitivity to amiloride suggests that it operates independent of K^+^/H^+^ exchange. Reduction of cellular ATP with NaN~3~ and KCN inhibits K^+^/H^+^ exchange showing thereby that the process is energy dependent. The K^+^/H^+^ exchange is sensitive to inhibitors of the gastric K^+^/H^+^‐ATPase. It is concluded that the H^+^‐ATPase previously reported on the plasma membrane of L. donovani is in fact a K^+^/H^+^‐ATPase. © 1994 wiley‐Liss, Inc.