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Plasma kinetic study of folinic acid and 5-methyltetrahydrofolate in healthy volunteers and cancer patients by high-performance liquid chromatography

✍ Scribed by Brigitte Payet; Gérard Fabre; Nicole Tubiana; Jean-Paul Cano

Book ID
104689127
Publisher
Springer
Year
1987
Tongue
English
Weight
652 KB
Volume
19
Category
Article
ISSN
0344-5704

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An automatic on-line high-performance liquid chromatographic (HPLC) method is developed to quantitate free amino acids in the biological samples. The method is based on pre-column derivatization of amino acids with orthophthalaldehyde (OPA) in presence of 2-mercaptoethanol (2-ME). The derivatized am