Plasma arginase concentration measured by an enzyme-linked immunosorbent assay (ELISA) in normal adult population
✍ Scribed by Soo-Ray Wang; Mei-Lin Chen; Ming-Ho Huang; Hsiao-Yi Lin; Jaw-Ji Tsai; Benjamin Ing-Tiau Kuo
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 483 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0009-9120
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✦ Synopsis
Human liver arginase has many biological effects on lymphocytes, macrophages, liver cells, and tumor cells, in addition to its major role in the liver urea cycle. We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) method to quantitate arginase concentrations in plasma that can be applied to various body fluids. The sensitivity was 2.5 ng/mL. The coefficients of variation were good both in intra-and inter-assay. Using this method to study the stability of an arginase preparation, we found that plasma arginase was stable for only 1 or 2 days even at temperatures as low as 4 °C. The mean plasma level was 41.0 -+ 3.3 ng/mL(mean ± SE) in 143 normal subjects. There was no age difference in the general population and in the male group. However, in the female group, the plasma arginase level increased with age (p = 0.05). Its biological significance was unclear. As a whole, the ELISA method for the measurement of arginase concentration in the body fluid is convenient and reliable.