Plant transformation: a simple particle bombardment device based on flowing helium

✍ Scribed by Y. Takeuchi; M. Dotson; N. T. Keen

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 499 KB
• Volume: 18
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00020031

✦ Synopsis

We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.