Plant Stem Cells: Methods and Protocols

✍ Scribed by Muhammad Naseem, Thomas Dandekar

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 155
• Series: Methods in Molecular Biology 2094
• Edition: 1st ed. 2020
• Category: Library

✦ Synopsis

This volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.

✦ Table of Contents

Front Matter ....Pages i-xi
Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches (Aqib Syed, Anwar Hussain, Waheed Murad, Badshah Islam)....Pages 1-16
Bacterial Shoot Apical Meristem Inoculation Assay (Muhammad Naseem, Gökhan Gun, Ozge Osmanoglu, Fatima A. AlRemeithi, Jibran Iqbal, Thomas Dandekar)....Pages 17-22
Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana Using TAL Effector Nucleases (Joachim Forner)....Pages 23-30
Method to Study Gene Expression Patterns During De Novo Root Regeneration from Arabidopsis Leaf Explants (Jie Yu, Ning Zhai, Lin Xu, Wu Liu)....Pages 31-38
Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture Their Transcriptome Profile (Monika Mahajan, Ram Kishor Yadav)....Pages 39-47
Plant-Associated Microbes Alter Root Growth by Modulating Root Apical Meristem (Anwar Hussain, Husna , Ihsan Ullah, Muhammad Naseem)....Pages 49-58
Live Imaging of Arabidopsis Axillary Meristems (Bihai Shi, Hongli Wang, Yuling Jiao)....Pages 59-65
Molecular Modeling of the Interaction Between Stem Cell Peptide and Immune Receptor in Plants (Muhammad Naseem, Mugdha Srivastava, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi et al.)....Pages 67-77
Methods to Visualize Auxin and Cytokinin Signaling Activity in the Shoot Apical Meristem (Géraldine Brunoud, Carlos S. Galvan-Ampudia, Teva Vernoux)....Pages 79-89
Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-seed Vascular Plants, Using EdU Labeling (Rieko Fujinami)....Pages 91-99
Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thaliana (Aleksandra Sapala, Richard S. Smith)....Pages 101-112
Mapping a Transcriptome-Guided Arabidopsis SAM Interactome (Muhammad Naseem, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi, Martin Kaltdorf et al.)....Pages 113-118
3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox (Viktoriya V. Lavrekha, Taras Pasternak, Klaus Palme, Victoria V. Mironova)....Pages 119-125
Micropropagation of Rosaceous Species SAM Grown in Temperate Climate (Jiri Sedlak, Frantisek Paprstein)....Pages 127-136
A New Perspective on Cryotherapy: Pathogen Elimination Using Plant Shoot Apical Meristem via Cryogenic Techniques (Ergun Kaya, Selin Galatali, Sevinc Guldag, Onur Celik)....Pages 137-148
Back Matter ....Pages 149-150

✦ Subjects

Life Sciences; Plant Anatomy/Development; Stem Cells