Plant regeneration through callus initiation from anthers and ovules of Scabiosa columbaria
✍ Scribed by Gaea Romeijn; André A.M. van Lammeren
- Book ID
- 110233182
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 556 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0167-6857
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Production of callus from anthers of D. purpurea was obtained on several basal media supplemented with various amounts of auxins. Chromosome counts showed that the callus produced was haploid when the anthers 1) were of a dark-brown to black color, and 2) were cultured in the late tetrad stage of mi
Anthers from rice (Oryza sativa L.) subspecies japonica initiated more callus than their indica or indica x japonica counterparts. A mild stress, either by slow desiccation or heat shock, prior to the plating of anthers enhanced the ability to initiate callus. Slow dessication of anthers enhanced th