Plant regeneration of mesophyll protoplasts from a cytoplasmic albino mutant ofLycopersicon esculentumcv. Large Red Cherry
✍ Scribed by F. H. M. Derks; A. Zelcer; C. M. Colijn-Hooymans
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 1017 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Mesophyll protoplasts from in vitro grown plants of a cytoplasmic albino mutant of Lycopersicon esculentum cv. Large Red Cherry were isolated with yields between 0.4 to 4.4 x 106 protoplasts per gram leaf tissue. Success in the culture of these protoplasts was dependent on embedding of the protoplasts in 100 pl agarose droplets 0.6% (w/v). A plating efficiency of 4.0% was obtained when the protoplasts were cultured in TM-2 medium with sucrose concentrations of 8.7 to 9.6% (w/v) resulting in an osmotic pressure of 432 to 469 mOsmol kg -1 . After 14 days of protoplast culture, microcalli with a diameter of 3 mm were observed. After 3 weeks, macrocalli were obtained which were transferred to regeneration medium. Regeneration of shoot primordia, with a frequency of 19%, was obtained on TM-4 medium supplemented with 1% (w/v) sucrose. The first shoot primordia were visible 10 weeks after protoplast plating. For development of the shoot primordia into shoots it was necessary to increase the sucrose concentration to 6% (w/v). Eight out of eleven regenerants were diploid (2n = 2x = 24); the other three were tetraploid. Efficient regeneration of mesophyll albino protoplasts from tomato opens the way to select at the cellular level for the chloroplast transfers.