Plant regeneration fromin vitrocultures of cotyledon explants and anthers ofSinapis albaand its implications on breeding of crucifers
✍ Scribed by R. K. Jain; Ulrike Brune; W. Friedt
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 786 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
✦ Synopsis
Protocols for plant regeneration from cotyledon explant and anther cultures of Sinapis alba have been developed for creating doubled-haploids and somaclonal variation . Among the several cultivars tested in this study, only 'Arda' responded well to in vitro plant regeneration both from anther-as well as cotyledoncultures . Multiple shoot formation in cotyledon explants, which always followed a brief callusing phase, was found to be the best on MS medium with ZEA (1 .0 mg/l) and NAA (0.1 mg/1) . Regeneration frequency declined sharply in the absence of auxin or presence of other cytokinins and/or auxin . The frequency of shoot regeneration also declined with reduction in the photoperiod to 16h . On MS + BAP (1 .0 mg/1) + NAA (1 .0 mg/1) medium, cotyledonary explants showed profuse callusing, which could regenerate shoots on high ZEA + low NAA/IAA medium . However, it declined with progressing time in culture . Anthers, excised from fresh as well as cold pretreated buds, cultured on 10% sucrose containing MS media with different hormonal constitution, developed calli and/or embryos . Initial culture temperature was important with embryogenesis occurring only in anthers cultured at 30° C for 3 weeks . A high temperature (35° C) treatment was lethal for both callus as well as embryo formation . While BAP + NAA 'and ZEA + NAA/IAA supported embryogenesis, further plant regeneration from anther-or embryo-callus could be achieved in ZEA + NAA/IAA media . Some of the regenerants flowered already in vitro and had small and sterile flowers . Cytological examination of some of the root differentiating calli indicated the presence of haploid as well as diploid cells . Shoots were rooted during prolonged incubation on the same medium or on transfer to MS (reduced)/ B5 + ZEA + NAA media .