Plant regeneration from stem and petiole protoplasts of sweet potato (Ipomoea batatas) and its wild relative,I. lacunosa
✍ Scribed by M. M. Belarmino; T. Abe; T. Sasahara
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 990 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
✦ Synopsis
A protoplast-to-plant regeneration system has been established for sweet potato (Ipomoea batatas (L.) Lam.) and its wild relative, L lacunosa L. Viable protoplasts, isolated from preplasmolyzed stems and petioles of in vitro-grown plants, were cultured on liquid MS (Murashige & Skoog 1962) medium that supported cell division and colony formation. Embryogenic calli of sweet potato were induced on agar-solidified MS medium supplemented with 3% (w/v) sucrose, 50 mg 1-1 casamino acids, 0.2-0.5 mg 1 -l 2,4-D, 1.0 mg 1-1 kinetin and 1.0 mg 1-1 ABA. On average, 3 plants were regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 3% (w/v) sucrose, 800 nag 1-1 glutamine, 2.0 mg 1 -l BA or 1.0 mg 1-1 kinetin and 1.0 mg 1-1 GA3. Embryogenic calli of I. lacunosa L. were initiated on semi-solid MS medium containing 0.2-0.5 mg 1-1 IAA and 1.0-2.0 mg 1-1 BA. An average of 5 plants was regenerated from a single sweet potato callus subcultured on semi-solid MS medium containing 0.5 or 1.0 mg 1-l GA3.