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Plant regeneration from leaf protoplasts ofSolanum virginianumL. (Solanaceae)

✍ Scribed by L. Borgato; F. Pisani; A. Furini


Book ID
106544911
Publisher
Springer
Year
2007
Tongue
English
Weight
194 KB
Volume
88
Category
Article
ISSN
0167-6857

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Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 ixM BA, 2.6 p~M NAA and 2.2 p~M 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lin

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A procedure for the culture of Solanum etuberosum mesophyll protoplasts with subsequent shoot regeneration is described. Several factors affected protoplast yield, colony formation, and shoot regeneration from in vitro plants. A protoplast isolation medium with 0.6 M sucrose produced twice the yield