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Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri)

✍ Scribed by N. Kuchuk; R. G. Herrmann; H.-U. Koop

Book ID
106010635
Publisher
Springer
Year
1998
Tongue
English
Weight
245 KB
Volume
17
Category
Article
ISSN
0721-7714

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Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 ixM BA, 2.6 p~M NAA and 2.2 p~M 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lin