β Scribed by George, Edwin F.; Hall, Michael A.; Klerk, Geert-Jan De
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Following In The Footsteps Of Its Much-acclaimed First Edition, Plant Cell Culture Protocols, Second Edition Has Been Expanded And Revised To Include The Most Up-to-date And Frequently Used Techniques For Plant Cell And Tissue Culture. Readily Reproducible And Extensively Annotated, The Methods Rang
Culture conditions for high frequency somatic embryogenesis and plant regeneration from cotyledonary explants of Codonopsis lanceolata are described. The maximum induction frequency of somatic embryos from cotyledonary explants was 80% on Murashige and Skoog (MS) medium containing 6% sucrose with 1
Hypocotyl segments of 2- to 3-week-old radish (Raphanus sativus L. cv. F1 Handsome Fall) seedlings produced yellowish compact calli when cultured on Murashige and Skoog's (MS) medium supplemented with 1 mgl(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Upon transfer onto medium containing 6-benzyladen