Plant Genome Editing Technologies: Speed Breeding, Crop Improvement and Sustainable Agriculture (Interdisciplinary Biotechnological Advances)

✍ Scribed by Jen-Tsung Chen (editor), Sunny Ahmar (editor)

• Publisher: Springer
• Year: 2024
• Tongue: English
• Leaves: 316
• Category: Library

✦ Synopsis

This book reviews all important aspects of plant genome editing to shed new light on these genome editing technologies together in crop improvement and sustainable agriculture. The book is divided into three areas: the first of which reinterprets plant genome studies from an overview perspective, examining the safety and risk assessment of plants as well as breeding-related outcomes. The second area discusses recent advances in the understanding of crop improvement. A wide range of plant stress tolerance in relation to plant genome editing is then addressed, before turning attention to specific species approaches including rice, maize, wheat, and soybean. The entire book is devoted to an updated knowledge of plant genome editing technologies from all aspects of plant biology and agronomy as we move toward advancements in sustainable life research. The book will be highly recommended for agriculture biologists, agronomists, plant pathologists, and all related research areas.

✦ Table of Contents

Preface
Contents
Plant Genome Editing Technologies: An Updated Overview
1 Introduction
2 Genome-Editing Tools
3 Zinc-Finger Nucleases (ZFNs)
4 Transcription Activator-like Effector Nucleases (TALENs)
5 Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR/Cas9)
6 Applications of Genome-Editing Techniques in Agriculture
7 Improving the Abiotic Stress Tolerance of Plants
8 Improving the Biotic Stress Tolerance of Plants
9 Enhancing the Nutritional Quality of Plants
10 Improving Yield and the Physical Appearance of Plants
11 Challenges and Strategies Associated with Genome-Editing Techniques
12 Conclusion
References
Navigating the Path from Lab to Market: Regulatory Challenges and Opportunities for Genome Editing Technologies for Agriculture
1 Introduction
2 CRISPR/Cas Gene Editing
3 Examples of Application of New Breeding Technologies (NBTs)
4 Ensuring the Biosafety of Products Generated by New Breeding Techniques (NBTs)
5 Minimizing the Impact of the Term GMO
6 Regulation of CRISPR Methods
6.1 Introduction
6.2 Regulating Genetically Modified (GM) Crops Versus Genetically Edited (GE) Crops
6.3 Regulatory Approaches in Different Countries
6.3.1 The United States
6.3.2 Canada
6.3.3 Brazil
6.3.4 Argentina
6.3.5 European Union
7 Regulation of Different Genome Editing Techniques: How Regulations Differ for Techniques like CRISPR/Cas and Others
8 Ethical and Safety Concerns Related to CRISPR
9 Perspectives
10 Final Considerations
References
Novel Genome-Editing Approaches for Developing Non-GM Crops for Sustainable Improvement and the Mitigation of Climate Changes
1 Introduction
2 Base Editors and Prime Editors
3 Genetic Segregation for Eliminating Transgenic Sequences
4 Mobile CRISPR Grafting Strategy
5 Virus-Induced Genome Editing
6 Transient Expression of CRISPR/Cas Ribonucleoproteins (RNPs)
7 Morphogenic Transcription Factor Mediated to Accelerating Genome Edited
8 CRISPR-Combo
9 Conclusion
References
Genome-Editing Technologies in Crop Improvement
1 Introduction
2 Genome-Editing Technologies
2.1 Zinc-Finger Nucleases (ZFNs)
2.2 Transcription Activator-Like Effector Nucleases (TALENs)
2.3 Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated (Cas9)
3 Applications
3.1 Abiotic Stress
3.2 Biotic Stress
3.3 CRISPR/Cas9 System for Crop Improvement
3.4 Improvement of a Biosynthetic Pathway Through Genome Editing
4 Challenges
5 Conclusion
References
Plant Breeding Becomes Smarter with Genome Editing
1 Introduction
2 Relevance of Genome Editing in Plant Breeding for Removing Bottlenecks and Increasing Preciseness
3 CRISPR/Cas-Mediated GE and Its Comparison with Conventional Mutagenesis Techniques
4 Genome Editing for Enhancing Yield
4.1 Developing Stable Male Sterile Lines to Facilitate Hybrid Breeding
4.2 Fixation of Heterosis and Developing Apomictic Hybrids
4.3 Generating Novel Cis-Alleles in the Promoter to Generate a Gradient of Phenotypes
5 Expanding Breeder’s Toolbox
5.1 Doubled Haploids (DHs) and Haploid Inducer Lines
5.2 Haploid Inducer-Mediated Genome Editing
5.3 De Novo Domestication
5.4 Herbicide Tolerance
5.5 Chromosome Engineering
6 Conclusion
References
Plant Breeding Using the CRISPR-Cas9 System for Food Security and Facing Climate Change
1 Introduction
2 Challenges in Plant Breeding for Crop Improvement
3 CRISPR/Cas9 System as a Facilitating Tool for Plant Breeding
4 History of the CRISPR-Cas System
5 Phases of the CRISPR-Cas System
5.1 Adaptation
5.2 crRNA Processing
5.3 Interference
6 Structure of CRISPR Locus and Classification of Cas Proteins
7 Classification of the CRISPR-Cas System
8 Application of CRISPR/Cas9 System in Plant Breeding for Crop Improvement
8.1 Improvement in Yield and Yield-Related Traits
8.2 CRISPR/Cas9 in Plant Hybrid Breeding
8.3 CRISPR/Cas9 in Apomictic Breeding
9 Development of Climate-Resilient Crops
9.1 Drought Stress Tolerance/Resistance
9.2 Salt Stress Tolerance/Resistance
9.3 Cold Stress Tolerance/Resistance
10 Heavy Metals Tolerance/Resistance
10.1 Herbicide Tolerance
10.2 Development of Disease-Resistant Crops
10.3 De Novo Domestication of Crop Wild Relatives and Orphan Crops
11 Limitations of CRISPR-Cas
11.1 Off-Target Effects of CRISPR Technology
11.2 Protospacer Adjacent Motif Requirement
11.3 DNA-Damage Toxicity
11.4 Delivery of CRISPR Tools
11.5 Toxicity and Immunogenicity of Cas Proteins
11.6 Target Site Restriction
11.7 Sensitivity to RNA Secondary Structure
11.8 RNA Instability and Occurrence of Mosaicism
11.9 Immunotoxicity
11.10 Precision Gene Editing with CRISPR
11.11 Delivery of CRISPR Gene Therapy
12 Future Perspectives
13 Conclusion
References
Plant Genome Editing for Enhanced Biotic Stress Tolerance Using the CRISPR/Cas Technology
1 Introduction
2 CRISPR/Cas Technology
2.1 The Cas Protein
2.1.1 Cas9
2.1.2 Cas12
2.1.3 Cas13
2.2 Nucleic Acid Repair Mechanism
2.2.1 Homology-Directed Repair (HDR)
2.2.2 Nonhomologous End Joining (NHEJ)
3 Omics-Based CRISPR/Cas-Mediated Plant Genome Editing
3.1 Genomics
3.2 Proteomics
3.3 Metabolomics
3.4 Transcriptomics
4 Gene Alteration to Improve Biotic Stress Tolerance Using Cas Protein
4.1 Resistance to Bacteria Through CRISPR/CAS-Mediated Editing
4.2 CRISPR/CAS Mediated Fungal Pathogen Resistivity
4.3 Viral Resistance Using CRISPR/Cas Technology in Plants
4.4 Resistance to Insect Infection by CRISPR/Cas System in Plants
4.5 CRISPR/Cas-Based Modification to Increase Nematode Resistance
4.6 Weed Resistance by CRISPR/Cas-Mediated Gene Editing
5 Future Perspective
6 Conclusion
References
The Application of Genome Editing Technologies in Soybean (Glycine max L.) for Abiotic Stress Tolerance
1 Introduction
2 CRISPR-Cas9 Role in Salinity Stress
3 CRISPR-Cas9 Role in Drought Stress
4 CRISPR-Cas9 Role in Heat Stress
5 CRISPR-Cas9 Role in Heavy Metal Stress
6 Other Abiotic Stress
7 Predicament and Solution of Genome Editing Technology in Soybean
References
Improving Qualities of Horticultural Crops Using Various CRISPR Delivery Methods
1 Introduction
2 Advanced Genome Editing by CRISPR Systems and Current Delivery Methods
2.1 CRISPR-Cas Nucleases and Their Variants
2.2 Delivery of CRISPR-Cas Components into Plant Cells
2.2.1 Protoplast Transformation Using PEG-Mediated CRISPR/Cas9 Delivery
2.2.2 Agrobacterium-Mediated CRISPR/Cas9 Delivery
2.2.3 Biolistics or Particle Bombardment
2.2.4 Viral Vectors
2.2.5 Nanocarriers
3 Genome Editing in Vegetables and Fruits
4 Genome Editing in Ornamental Crops
5 Challenges and Future Perspectives for the Improvement of Horticulture Crops Through Genome Editing
References
CRISPR/Cas Genome Editing in Fruit Crops: Recent Advances, Challenges, and Future Prospects
1 Introduction
2 Components and Mechanisms of Action
3 Application of CRISPR/Cas in Fruit Crops
3.1 Nutritional Improvements
3.2 Postharvest Loss
3.3 Biotic Stress
3.4 Abiotic Stress
3.5 Plant Growth and Development
3.6 Other Traits
4 Challenges
5 Future Perspectives and Conclusion
References
Plant Tissue Culture: A Boon or Enigma in Gene Editing for Plants Using CRISPR/Cas System
1 Introduction
2 Genome Editing Techniques with Special Reference to CRISPR
2.1 SSRs-Mediated Genome Engineering
2.2 SSNs-Operated Genome Editing
2.2.1 Meganucleases-Associated Editing
2.2.2 ZFNs-Mediated Editing
2.2.3 TALENs-Induced Modifications
2.2.4 CRISPR/Cas9-Based Gene Editing
3 PTC (Plant Tissue Culture) and CRISPR/Cas9
4 Conclusion
References
The Use of Gene Editing Technology to Introduce Targeted Modifications in Woody Plants
1 Introduction
2 Need for Genome Modification in Woody Plants
3 Mechanism of Genomic Editing Tools
3.1 Comparison Among Genome Editing Tools
3.2 Mechanism of the CRISPR/Cas9 Nuclease System
4 Challenges for Genome Engineering of Woody Plants
5 Significance of CRISPR in Genome Editing of Woody Plants
6 Conclusion and Prospects
References
Single-Base Editing in the Arabidopsis SUMO Conjugating Enzyme by Adenine Base Edition and Screening for a Rare Editing Event
1 Biological Background
2 Constructs for Site-Directed Mutagenesis
3 Control of Efficiency by Cas9 Without Deaminase
4 Assessment of Efficiency: Somatic Mutation Rate and Heat Treatment
5 Screening Scheme: Pooling of Leaf Material in Search of Heritable Changes
6 Sequence Interpretation
7 De-convolution of Positive Pools: Identification of Mutated Plants
8 Summary
References