Tools For Expressing Foreign Genes In Plants / François Guerineau -- Introduction Of Cloning Plasmids Into Agrobacterium Tumefaciens / Charles H. Shaw -- Leaf Disk Transformation Using Agrobacterium Tumefaciens : Expression Of Heterologous Genes In Tobacco / Patrick Gallois, Paulo Marinho -- Agobacterium Rhizogenes As A Vector For Transforming Higher Plants : Application In Lotus Corniculatus Transformation / Jens Stougaard -- Agrobacterium-mediated Transformation Of Arabidopsis Thaliana : Application In T-dna Tagging / Jennifer F. Topping, Wenbin Wei, Michael C. Clarke, Paul Muskett, Keith Lindsey -- Agrobacterium-mediated Transfer Of Geminiviruses To Plant Tissues / Margaret I. Boulton -- Stable Transformation Of Barley Via Direct Dna Uptake : Electroporation- And Peg-mediated Protoplast Transformation / Paul A. Lazzeri -- Gene Transfer Into Plant Protoplasts By Electroporation / Heddwyn Jones --^ Transformation Of Cereals By Microprojectile Bombardment Of Immature Inflorescence And Scutellum Tissues / Pilar Barcelo, Paul A. Lazzeri -- The [beta]-glucuronidase (gus) Reporter Gene System : Gene Fusions : Spectrophotometric, Fluorometric, And Histochemical Detection / Gillian A. Hull, Martine Devic -- Chloramphenicol Acetyl Transferase Assay / Michael R. Davey, Nigel W. Blackhall, J. Brian Power -- Nptii Assays For Measuring Gene Expression And Enzyme Activity In Transgenic Plants / Ian S. Curtis, J. Brian Power, Michael R. Davey -- Gene Characterization By Southern Analysis / Paolo A. Sabelli, Peter R. Shewry -- Isolation And Characterization Of Plant Genomic Dna Sequences Via (inverse) Pcr Amplification / Remko Offringa, Frederique Lee -- Isolation Of Whole Cell (total) Rna / Rod J. Scott -- Poly(a)+ Rna Isolation / Rod J. Scott -- In Vitro Translation / Rod J. Scott -- Northern Analysis And Nucleic Acid Probes / Paolo A. Sabelli, Peter R. Shewry --^ Nuclear Run-on Transcription Assays / John Gatehouse, Andrew J. Thompson -- Rnase A/t1 Protection Assay / Craig G. Simpson, John W. Brown -- Primer Extension Assay / Craig G. Simpson, John W. Brown -- Applications Of Rt-pcr / Craig G. Simpson, John W. Brown -- In Vitro Transcription Of Class Ii Promoters In Higher Plants / Richard Cooke, Paul Penon -- Analysis Of Plant Gene Expression By Reverse Transcription-pcr / Mark Bell -- In Situ Hybridization To Plant Tissue Sections / Shirley R. Burgess -- Xenopus Oocytes As A Heterologous Expression System / Frederica L. Theodoulou, Anthony J. Miller -- Heterologous Expression In Yeast / Laurence J. Trueman -- The Isolation Of Intact Chloroplasts / Johnathan A. Napier, Simon A. Barnes -- In Vitro Protein Import By Isolated Chloroplasts / Johnathan A. Napier -- Targeting Of Foreign Proteins To The Chloroplast / Johnathan A. Napier -- Isolation Of Mitochondria / Stefan Binder, Lutz Grohmann --^ Mitochondrial Nucleic Acid Purification And Analysis / Stefan Binder -- In Organello Protein Synthesis / Lutz Grohmann -- Separation Of Plant Proteins By Electrophoresis / Peter R. Shewry, Arthur S. Tatham, Roger J. Fido -- Western Blotting Analysis / Roger J. Fido, Arthur S. Tatham, Peter R. Shewry -- Elisa Detection Of Foreign Proteins / E.n. Mills, Geoffrey W. Plumb, Michael R. Morgan -- Immunocytochemical Localization Of Proteins / John Davies. Edited By Heddwyn Jones. Includes Bibliographical References And Index.