Plant Cryopreservation: A Practical Guide

✍ Scribed by Barbara M. Reed (auth.), Barbara M. Reed (eds.)

• Publisher: Springer-Verlag New York
• Year: 2008
• Tongue: English
• Leaves: 533
• Edition: 1
• Category: Library

✦ Synopsis

Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.

✦ Table of Contents

Front Matter....Pages i-xxix
Cryopreservation—Practical Considerations....Pages 3-13
Cryopreservation Theory....Pages 15-32
Development of PVS-Based Vitrification and Encapsulation–Vitrification Protocols....Pages 33-57
Development of Encapsulation Dehydration....Pages 59-75
Controlled Rate Cooling....Pages 77-92
Cryopreservation of Algae....Pages 95-116
Cryopreservation of Bryophytes and Ferns....Pages 117-140
Cryopreservation of Dedifferentiated Cell Cultures....Pages 141-176
Cryopreservation of Embryogenic Cultures....Pages 177-210
Cryopreservation of Excised Embryos and Embryonic Axes....Pages 211-240
Cryopreservation of Monocots....Pages 241-280
Cryopreservation of Herbaceous Dicots....Pages 281-332
Cryopreservation of Temperate Berry Crops....Pages 333-364
Cryopreservation of In Vitro Tissues of Deciduous Forest Trees....Pages 365-386
Cryopreservation of Fruit and Ornamental Trees....Pages 387-420
Cryopreservation of Dormant Buds....Pages 421-442
Cryopreservation of Pollen....Pages 443-464
Cryopreservation of Recalcitrant (i.e. Desiccation-Sensitive) Seeds....Pages 465-484
Cryopreservation of Orthodox (Desiccation Tolerant) Seeds....Pages 485-501
Back Matter....Pages 503-513

✦ Subjects

Plant Genetics & Genomics; Plant Sciences; Agriculture