Affinity-purified, fluorochrome-tagged F(ab′) 2 antibody fragments specific for heavy (µ) chains of Rana pipiens IgM were prepared from hyperimmune rabbit sera. By using two-color immunofluorescent procedures we observed that (1) the first cells expressing IgM, termed pre-B cells, lack detectable qu
✦ LIBER ✦
Pineal regeneration in the frog, Rana pipiens, following embryonic extirpation
✍ Scribed by Victor B. Eichler
- Publisher
- John Wiley and Sons
- Year
- 1968
- Tongue
- English
- Weight
- 340 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0362-2525
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✦ Synopsis
This report describes the capacity of larvae of the Leopard frog, R u m pipiens, to regenerate pineal tissue after embryonic pinealectomy, and presents experimental evidence for frontal organ dependence on the epiphysis. In addition, it was found that the "brow spot," a pigment-free region located in the epidermis between the eyes, is dependent on the frontal organ for its formation. This report also substantiates that the absence of the pineal organ in larval animals does not affect growth o r development through metamorphosis.
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