Pine needle abortion in cattle: analysis of isocupressic acid in North American gymnosperms
✍ Scribed by Dale R. Gardner; Lynn F. James
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 56 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Isocupressic acid is a labdane resin acid found in Pinus ponderosa needles and causes abortion in late term pregnant cattle. A chemical survey was conducted to determine the phytochemical distribution of isocupressic acid in selected North American shrub and tree species with possible association with cattle abortions. A method of analysis for the evaluation of total available isocupressic acid (including hydrolysed acetyl and succinyl derivatives) was developed based on a base/acid extraction of dry, ground plant material, followed by detection and quantitative determination using capillary gas chromatography of the di-(trimethylsilyl)-derivative. Samples from 23 different species were collected throughout the western and southern regions of the United States and analysed. Isocupressic acid was detected in 15 species, and significant concentrations (b0.5% dry weight) were found in Pinus ponderosa, P.