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Pim-3 is expressed in endothelial cells and promotes vascular tube formation

✍ Scribed by Peng Zhang; Hanqin Wang; Xinwen Min; Yinfang Wang; Jie Tang; Jishun Cheng; Dongfeng Li; Xin Chen; Fanjun Cheng; Nanping Wang; Handong Yang

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
425 KB
Volume
220
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Pim‐3 is a member of proto‐oncogene Pim family that encodes serine/threonine kinases. Pim proteins regulate both apoptosis and cellular metabolism by phosphorylating their substrates. Here, we report for the first time that Pim‐3 is highly expressed at mRNA and protein levels in endothelial cells (ECs). We found that Pim‐3 is concentrated at the cellular lamellipodia and co‐localized with focal adhesion kinase (FAK). Pim‐3 was dispersed from lamellipodia when ECs were treated with cytochalasin D, an inhibitor of actin polymerization. In addition, small‐interfering RNA (siRNA)‐mediated gene knockdown of Pim‐3 significantly impaired EC spreading, migration, and proliferation, leading to a reduction in tube‐like structure formation in a Matrigel assay. These results provide the novel evidence that Pim‐3 plays an essential role in EC spreading and migration, suggesting that Pim‐3 may be an important molecular target for the development of small‐molecule inhibitors of angiogenesis. J. Cell. Physiol. 220: 82–90, 2009. © 2009 Wiley‐Liss, Inc.

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