Pilot-scale production of (S)-styrene oxide from styrene by recombinant Escherichia coli synthesizing styrene monooxygenase

Production of enantiopure styrene oxide

Production of enantiopure styrene oxide by recombinant Escherichia coli synthesizing a two-component styrene monooxygenase

✍ Sven Panke; Marcel G. Wubbolts; Andreas Schmid; Bernard Witholt 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 268 KB 👁 1 views

A whole cell biocatalytic process was developed to enable the efficient oxidation of styrene to chiral (S)-styrene oxide with an enantiomeric excess better than 99%. Recombinant Escherichia coli cells were employed to express the genes styAB encoding the styrene monooxygenase of Pseudomonas sp. stra