## Abstract In denuded and cumulusβenclosed pig oocytes, puromycin at concentrations 5, 10, and 25 ΞΌg/ml did not lower the rate of germinal vesicle breakdown (GVBD) after 24 h of culture. GVBD was prevented in 50, 75, and 100 ΞΌg/ml of puromycin. After 40 h of culture, 5 and 10 ΞΌg puromycin/ml impai
Pig membrana granulosa cells prevent resumption of meiosis in cattle oocytes
β Scribed by Jaroslav Kalous; Peter Sutovsky; Zora Rimkevicova; Yasuo Shioya; Byong-Lyul Lie; Jan Motlik
- Book ID
- 111662412
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 921 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1040-452X
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## Abstract The role of calcium (Ca^+ +^) during spontaneous meiotic maturation of pig oocytes was examined. The hypothesis that elevations of endogenously derived intracellular Ca^+ +^ are prerequisite for germinal vesicle breakdown (GVBD) and progression through meiosis was tested. In addition, i
## Abstract A factor, secreted by theca cells, inhibits FSH induced resumption of meiosis in bovine oocytes that are surrounded by cumulus cells which are attached to a piece of the membrana granulosa (COCGs). In order to characterize this factor, theca cell conditioned medium (CMt) was heatβtreate