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Pig liver fatty acid synthetase: Purification and physicochemical properties

โœ Scribed by In-Cheol Kim; Clifford J. Unkefer; William C. Deal Jr.


Book ID
115703302
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
1012 KB
Volume
178
Category
Article
ISSN
0003-9861

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Fatty acid synthetase has been purified from Cryptococcus neoformans 450 fold to a specific activity of 3.6 units per mg protein with an overall yield of 23%. The purified enzyme contained two non-identical subunits, Mr approximately 2.1 x 105 and 1.8 x 105. Under optimum conditions, 100 mM KC1 and