Piezoelectric immuno sensor for the detection of candida albicans microbes

✍ Scribed by H. Muramatsu; K. Kajiwara; E. Tamiya; I. Karube

• Book ID: 104100385
• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 318 KB
• Volume: 188
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(00)86049-3

✦ Synopsis

The system consists of an AT-cut quartz piezoelectric crystal, oscillator and frequency counter. The surface of the palladium-plated electrodes is oxidized anodically, and anti-Candida antibody is immobilized onto the surface. The crystal sensor is dipped into Candida suspension and the surface mass increase, caused by immuno-adsorption of Candida, is measured by the decrease in the resonant frequency of the crystal. The frequency shift is correlated with C. albicans concentration in the range 106-5 X IO' cells cm-3. The crystal sensor showed no response to Saccharomyces cerevisiae.