Piezoelectric crystal biosensor modified with protein A for determination of immunoglobulins

✍ Scribed by Muramatsu, Hiroshi.; Dicks, Jonathan M.; Tamiya, Eiichi.; Karube, Isao.

• Book ID: 120034114
• Publisher: American Chemical Society
• Year: 1987
• Tongue: English
• Weight: 483 KB
• Volume: 59
• Category: Article
• ISSN: 0003-2700
• DOI: 10.1021/ac00150a007

✦ Synopsis

Determlnatlon of lmmunoglobullns was performed by uslng a piezoelectric crystal bhensor system. AT-cut crystals, wlth a baslc resonant frequency of 0 YHr, were modlfled by Immobillring Protein A onto the surface of crystal electrodes wlth ( y-am1nopropyl)trlethoxysllane. The crystal was posi- tloned lnslde a thennostated How cell and measurement of the resonant frequency was performed In constantly flowlng delonlzed/dlstilled water. The resonant frequency shlft resultlng from the afflnlty reactlon of Protein A and human IgG correlated wlth a concentration of human IgG In the range 10a-10-2 mg-mL-'. The analysis pattern of mouse IgG subclasses, obtalned by uslng a stepped gradient of buffer solution, ldentlfied three dlstlnctlve peaks of IgG,, IgG,,, and IgG2b.