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Picosecond Time-Resolved FRET in the Fluorescent Protein from Discosoma Red (wt-DsRed)

✍ Scribed by Tanja A. Schüttrigkeit; Ulrich Zachariae; Till von Feilitzsch; Jens Wiehler; Julia von Hummel; Boris Steipe; Maria E. Michel-Beyerle

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
87 KB
Volume
2
Category
Article
ISSN
1439-4235

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✦ Synopsis

Ultrafast, intra-oligomer fluorescence resonance energy transfer (FRET) between an immature green-emitting GFP-like chromophore to the mature red-emitting chromophore is found in the novel red fluorescent protein wt-DsRed (the picture shows the steady-state absorption (solid line) and emission (dotted) spectra). Since FRET is by its very nature a short range process, it represents a highly suitable method to probe oligomerization. This work describes a method preferentially applicable to the efficient screening of protein variants with mutagenetically altered surface docking sites.

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