Picorna- and togavirus infection of cells detected by gas chromatography

✍ Scribed by Dr. A. Levanon; Y. Klibansky; A. Kohn

• Book ID: 102905435
• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 517 KB
• Volume: 1
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890010310

✦ Synopsis

Abstract

Viral infection of cells causes chemical and metabolic changes, which can be detected by gas chromatography (GC) of ether extracts of supernatant fluids and cell homogenates. before any significant damage to the cells is observable microscopically. The characteristic and specific GC patterns obtained from BHK‐21 and Vero cell cultures infected with encephalomyocarditis, polio, echoviruses, and a togavirus make it possible to distinguish between these infecting viruses. The appearance of 1 or 2 compounds, represented by GC peaks with T~R~ values of 302 and 677 seconds seems to be specific for these viruses. Other peaks found in the supernatant media 1–2 hours after infection probably represent cell constituents leaking into the medium as a result of damage to the cell membrane by the invading virus.