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Picomolar Assay of Native Proteins by Capillary Electrophoresis Precolumn Labeling, Submicellar Separation, and Laser-Induced Fluorescence Detection

โœ Scribed by Pinto, Devanand M.; Arriaga, Edgar A.; Craig, Doug; Angelova, Jordanka; Sharma, Neepun; Ahmadzadeh, Hossein; Dovichi, Norman J.; Boulet, Camille A.

Book ID
120567025
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
285 KB
Volume
69
Category
Article
ISSN
0003-2700

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Py-1 and Py-6 are novel amino-reactive fluorescent reagents. The names given to them reflect that they consist of a pyrylium group attached to small aromatic moieties. Upon reaction with a primary amine there is a large spectral shift in the reagent, rendering them effectively fluorogenic. In this s