✦ LIBER ✦

Picoliter 1H NMR Spectroscopy

✍ Scribed by Kevin R. Minard; Robert A. Wind

Publisher: Elsevier Science
Year: 2002
Tongue: English
Weight: 215 KB
Volume: 154
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.2001.2494

No coin nor oath required. For personal study only.

✦ Synopsis

In this study, a 267-µm-diameter solenoid transceiver is used to acquire localized 1 H NMR spectra and the measured signal-to-noise ratio (SNR) at 500 MHz is shown to be within 20-30% of theoretical limits formulated by considering only its resistive losses. This is illustrated using a 100-µm-diameter globule of triacylglycerols (∼900 mM) that may be an oocyte precursor in young Xenopus laevis frogs and a water sample containing choline at a concentration often found in live mammalian cells (∼33 mM). In chemical shift imaging (CSI) experiments performed using a few thousand total scans, the choline methyl line is shown to have an acceptable SNR in resolved volume elements containing only 50 pL of sample, and localized spectra are resolved from just 5 pL in the Xenopus globule. These findings demonstrate the feasibility of performing 1 H NMR on picoliter-scale sample volumes in biological cells and tissues and illustrate how the achieved SNR in spectroscopic images can be predicted with reasonable accuracy at microscopic spatial resolutions.

📜 SIMILAR VOLUMES

3H1H shift correlation NMR spectroscopy

✍ Carel W. Funke; Gerard N. Wagenaars; Frans M. Kaspersen 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 402 KB

Three two-dimensional 'H-lH chemical shift correlation experiments are described, all using familiar pulse sequences. The experiments reveal the chemical shifts of the 'H spins which are coupled scalarly to a 'H spin and, thus, assist in the unambiguous location of this 'H spin within the molecule.

1H NMR Spectroscopy of pyrrolizidine alk

1H NMR Spectroscopy of pyrrolizidine alkaloids

✍ H.J. Segall; J.L. Dallas 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 291 KB

Single-shot diffusion trace 1H NMR spect

Single-shot diffusion trace 1H NMR spectroscopy

✍ Robin A. de Graaf; Kees P.J. Braun; Klaas Nicolay 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 219 KB

## Abstract Ignoring diffusion anisotropy can severely hamper the quantitative determination of water and metabolite diffusion in complex tissues. The measurement of the trace of the diffusion tensor provides unambiguous and rotationally invariant ADC values, but usually requires three separate exp

Localized double-quantum-filtered 1H NMR

Localized double-quantum-filtered 1H NMR spectroscopy

✍ M.A Thomas; H.P Hetherington; D.J Meyerhoff; D.B Twieg 📂 Article 📅 1991 🏛 Elsevier Science ⚖ 700 KB

Advanced 1H Solid-State NMR Spectroscopy

Advanced 1H Solid-State NMR Spectroscopy on Hydrogels, 1

✍ Eva Díez-Peña; Isabel Quijada-Garrido; José Manuel Barrales-Rienda; Ingo Schnell 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB

## Abstract **Summary:** The nature of the pH dependent collapse of poly(methacrylic acid) (PMAA) hydrogels is investigated using recent ^1^H solid‐state NMR methods. In aqueous solution, PMAA changes from an expanded conformation at high pHs to a compact contracted form at low pHs, where hydrogen

Estimation of liposome integrity by 1H-N

Estimation of liposome integrity by 1H-NMR spectroscopy

✍ N.N. Ivanov; S.V. Rykov; O.L. Isakova; E.K. Ruuge; V.P. Torchilin 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 377 KB

A fast and simple method of 1H-NMR spectroscopic control of liposome membrane integrity is suggested. The method is based on the redistribution of intensities between two singlet 1H-NMR signals--from intraliposomal marker compound (nitrilotriacetic acid sodium salt, 1H-NMR signal at 4 p.p.m.) and fr